Identification of T-Cell Antigens Specific for Latent Mycobacterium Tuberculosis Infection
نویسندگان
چکیده
منابع مشابه
Identification of T-Cell Antigens Specific for Latent Mycobacterium Tuberculosis Infection
BACKGROUND T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans. METHODOLOGY/PRINCIPAL FINDINGS We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tub...
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To the Editor: In their review article, Getahun et al. (May 28 issue)1 provide a useful overview of issues surrounding latent tuberculosis infection. However, newly published data regarding the performance of the T-SPOT.TB assay call into question the supposition that false conversions and reversions are more common with interferon-γ release assays (IGRAs) than with tuberculin skin tests.2 Spec...
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Abbreviations 1 1 Abbreviations This thesis uses the international one-letter amino acid code. ADC albumin, dextrose, catalase Ag85a/b Antigen85 a/b of M. tuberculosis Alexa# Alexa Fluor® # AmCyan Anemonia majano cyan fluorescent protein APC antigen presenting cell APC allophycoerythrin APC-Cy7 allophycoerythrinCychrome7 ATP adenosine tri-phosphate BAK BCG activated killer cells BAL bronchoalve...
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Although IFN-γ release assays (IGRAs) provide increased specificity over tuberculin skin tests, the early and sensitive detection of reactivation of latently infected Mycobacterium tuberculosis is required to control tuberculosis (TB). Recently, a multicolor flow cytometry has been developed to study CD4(+) T cell cytokine responses (IFN-γ/IL-2/TNF-α) to purified protein derivatives (PPD) and M...
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Development of a subunit vaccine for Mycobacterium tuberculosis (Mtb) depends on the identification of Ags that induce appropriate T cell responses. Using bioinformatics, we selected a panel of 94 Mtb genes based on criteria that included growth in macrophages, up- or down-regulation under hypoxic conditions, secretion, membrane association, or because they were members of the PE/PPE or EsX fam...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2009
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0005590